Effects of regional cerebral blood flow perfusion on learning and memory function and its molecular mechanism in rats

doi:10.18679/CN11-6030/R.2016.009

Translational Neuroscience and Clinics

2016, Vol. 2

Issue (1): 17-24 doi: 10.18679/CN11-6030/R.2016.009

Original Articles

Effects of regional cerebral blood flow perfusion on learning and memory function and its molecular mechanism in rats

Cunli Xu¹, Wenhua Wu², Lingbin Kong³

1 Rehabilitation Medicine Center, The First People's Hospital of Jining, Jining, China;
2 Department of Neurology, The Second People's Hospital of Jining, Jining, China;
3 Shandong Institute of Behavioral Medical Science, Jining Medical College, Jining, China

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Abstract Objective: To study the effects of regional cerebral blood flow (rCBF) perfusion on learning and memory function in special brain areas and its molecular mechanism in rat. Methods: Sixty-four adult male healthy Sprague-Dawley (SD) rats were randomly divided into two groups: A false operation group and an operation group. The false operation group was randomly divided into four subgroups (A₀, B₀, C₀, and D₀) and the operation group was randomly divided into four subgroups (A, B, C, and D), with eight rats in each subgroup. The operation group underwent bilateral common carotid artery permanent ligation, while the other group only underwent a skin incision without the bilateral common carotid artery permanent ligation. Learning memory function of rats in each subgroup was measured using a Y-maze at 4 h, 8 h, 24 h, and 3 days after surgery. The rCBF in the right frontal lobe and hippocampus was detected using the Periflux PF model laser Doppler flowmetry and c-fos, c-jun, Bcl-2, and Bax protein expression in the right frontal lobe and hippocampus was measured using immunohistochemistry. Results: The rCBF in the right frontal lobule division and right hippocampus division was significantly lower in the operation group than in the false operation group (P < 0.05). The error number (EN), time to reach the target, and total reaction time (TRT) for the learning index using the Y-type labyrinth test in the operation group were significantly higher than that in the false operation group (P < 0.05); however, the active avoid rate in the operation group was significantly lower than that of the false operation group. Expression of c-fos and c-jun as well as the average absorbency in the right frontal lobule division and right hippocampus division in the operation group were significantly higher than those in the false operation group (P < 0.05). The number of Bax and Bcl-2-positive cells was significantly higher in the operation group, and the expression ratio of Bax/Bcl-2 in the operation group was significantly higher than that in the false operation group (P < 0.01). Conclusions: rCBF decrease can impair learning and memory function in rats, which may be related to the increased expression of c-fos, c-jun, Bcl-2, and Bax proteins in the t he frontal cortex and hippocampus.

Key words： regional cerebral blood flow learning and memory function correlation molecular mechanism

Received: 11 January 2016 Published: 31 March 2016

Corresponding Authors: Lingbin Kong, E-mail: klb3904@163.com E-mail: klb3904@163.com


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Cunli Xu, Wenhua Wu, Lingbin Kong. Effects of regional cerebral blood flow perfusion on learning and memory function and its molecular mechanism in rats. Translational Neuroscience and Clinics, 2016, 2(1): 17-24.

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http://tnc.tsinghuajournals.com/10.18679/CN11-6030/R.2016.009 OR http://tnc.tsinghuajournals.com/Y2016/V2/I1/17

Table 1 Learning and memory performances between the operation and false operation groups at different times after operation

Table 2 The comparison of the rCBF (mL/(100g·min)) in the right frontal lobe and hippocampus between the operation and false operation groups at different times

Table 3 The comparison of the expression of c-fos and c-jun in the right hippocampus and frontal lobe of each group of rats after the operation

Table 4 The comparison of the average absorbency of c-fos, c-jun in the right hippocampus and frontal lobe of each group of rats after the operation

Table 5 The comparison of the expression of Bcl-2 and Bax in the right hippocampus and frontal lobe of the rats in the operation subgroups

Figure 1(a–b) c-fos protein positive cells in the hippocampus tissue of A0 and A subgroups (serosity SP dyed ×400).

Figure 2(a–b) c-jun protein positive cells in the hippocampus tissue of the A0 and A subgroups ( karyon SP dyed ×400)

Figure 3(a–b) Bcl-2 expression in the immunohistochemical slice from the hippocampus cell serosity (SP ×400).

Figure 4(a–b) Bax expression in the immunohistochemical slice from the hippocampus cell serosity (SP ×400).

Table 6 The comparison of Bax/Bcl-2 protein expression ratio in the right hippocampus and frontal lobe of the rats in the operation subgroups

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