Effect of heparin on the expressions of serum intercellular adhesion molecule-1 and hippocampus S100β protein after cardiopulmonary resuscitation in rabbits

doi:10.18679/CN11-6030/R.2016.038

Translational Neuroscience and Clinics

2016, Vol. 2

Issue (4): 227-230 doi: 10.18679/CN11-6030/R.2016.038

Original Articles

Effect of heparin on the expressions of serum intercellular adhesion molecule-1 and hippocampus S100β protein after cardiopulmonary resuscitation in rabbits

Wenxun Liu^1,2, Yun Wang^1,2, Xiaohong Zhou^1,2, Wenjuan Cheng², Qingshan Ye^1,2

1. Department of Anesthesia, Ningxia People's hospital, Yinchuan 750002, China;
2. The First Clinical Medical College of Northwest University for Nationalities, Yinchuan 750002, China

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Abstract Objective: Use heparin during cardiac arrest (CA) in rabbits and observe the serum intercellular adhesion molecule-1 (ICAM-1) and hippocampal S100β protein expressions after cardiopulmonary resuscitation (CPR).
Methods: Thirty-two New Zealand rabbits were randomly divided into, Group I, control group; Group II, saline group; Group III, heparin group. Each animal underwent continuous hemodynamic monitoring including mean arterial pressure (MBP), heart rate (HR), and the end-tidal carbon dioxide partial pressure (PetCO₂). Twenty-four hours after resuscitation, serum and hippocampal neurons were collected from all animals. Enzyme linked immunosorbent assay was used to detect serum ICAM-1 and immunohistochemistry to detect the S100β protein in hippocampal neurons. According to the rate of positive cells, each hippocampal specimen was categorized into four expression levels.
Results: The differences in the serum ICAM-1 concentration in the three groups were statistically significant. The expression of S100β protein in the hippocampus showed eight cases in group I at level 1 and none in groups II and III. There was 1 case in group II and 7 cases in group III at level 2; five cases in group II and 2 cases in group III at level 3; 2 cases in group II and 1 case in group III at level 4. The expression strength of S100β protein in the three groups differed significantly (P < 0.05).
Conclusions: Heparin therapy can reduce the expression of serum ICAM-1 and S100β protein in hippocampal neurons during CPR. It is possible that heparin can have a positive effect on brain protection during CPR.

Key words： cardiopulmonary-cerebral resuscitation heparin intercellular adhesion molecule-1 S100&beta protein

Received: 20 October 2016 Published: 30 December 2016

Corresponding Authors: Qingshan Ye,E-mail:yeqingshan@hotmail.com E-mail: yeqingshan@hotmail.com


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Wenxun Liu, Yun Wang, Xiaohong Zhou, Wenjuan Cheng, Qingshan Ye. Effect of heparin on the expressions of serum intercellular adhesion molecule-1 and hippocampus S100β protein after cardiopulmonary resuscitation in rabbits. Translational Neuroscience and Clinics, 2016, 2(4): 227-230.

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http://tnc.tsinghuajournals.com/10.18679/CN11-6030/R.2016.038 OR http://tnc.tsinghuajournals.com/Y2016/V2/I4/227


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