Preliminary analysis of cellular sociology of co-cultured glioma initiating cells and macrophages in vitro
Mingxia Zhang1, Junjie Chen2, Lin Wang1, Xiaoyan Ji1, Lin Yang1, Yujing Sheng1, Hairui Liu1, Haiyang Wang1, Aidong Wang1, Xingliang Dai1, Xiaonan Li3, Qiang Huang1, Jun Dong1
1 Department of Neurology, the Second Affiliated Hospital of Soochow University, Suzhou 215004, China;
2 Department of Neurology, Xishan People's Hospital, Wuxi 214000, China;
3 Laboratory of Molecular Neuron-oncology, Texas Children's Cancer Center, Baylor College of Medicine, Houston, TX, USA
Preliminary analysis of cellular sociology of co-cultured glioma initiating cells and macrophages in vitro
Mingxia Zhang1, Junjie Chen2, Lin Wang1, Xiaoyan Ji1, Lin Yang1, Yujing Sheng1, Hairui Liu1, Haiyang Wang1, Aidong Wang1, Xingliang Dai1, Xiaonan Li3, Qiang Huang1, Jun Dong1
1 Department of Neurology, the Second Affiliated Hospital of Soochow University, Suzhou 215004, China;
2 Department of Neurology, Xishan People's Hospital, Wuxi 214000, China;
3 Laboratory of Molecular Neuron-oncology, Texas Children's Cancer Center, Baylor College of Medicine, Houston, TX, USA
摘要 Objective: Real-time monitoring of cytokine secretion at the single immunocyte level, based on the concept of immune cells, sociology has been recently reported. However, the relationships between glioma-initiating cells (GICs) and host immune cells and their mutual interactions in the tumor microenvironment have not been directly observed and remain unclear. Methods: The dual fluorescence tracing technique was applied to label the co-cultured GICs and host macrophages (Mø), and the interactions between the two types of cells were observed using a live cell imaging system. Fusion cells in the co-culture system were monocloned and proliferated in vitro and their social interactions were observed and recorded. Results: Using real-time dynamic observation of target cells, 6 types of intercellular conjunction microtubes were found to function in the transfer of intercellular information between GICs and Mø; GICs and host Mø can fuse into hybrid cells after several rounds of mutual interactions, and then these fusion cells fused with each other; Fusion cells generated offspring cells through symmetrical and asymmetrical division or underwent apoptosis. A "cell in cell" phenomenon was observed in the fusion cells, which was often followed by cell release, namely entosis. Conclusions: Preliminary studies revealed the patterns of cell conjunction via microtubes between GICs and host Mø and the processes of cell fusion, division, and entosis. The results revealed malignant transformation of host Mø, induced by GICs, suggesting complex social relationships among tumor-immune cells in gliomas.
Abstract: Objective: Real-time monitoring of cytokine secretion at the single immunocyte level, based on the concept of immune cells, sociology has been recently reported. However, the relationships between glioma-initiating cells (GICs) and host immune cells and their mutual interactions in the tumor microenvironment have not been directly observed and remain unclear. Methods: The dual fluorescence tracing technique was applied to label the co-cultured GICs and host macrophages (Mø), and the interactions between the two types of cells were observed using a live cell imaging system. Fusion cells in the co-culture system were monocloned and proliferated in vitro and their social interactions were observed and recorded. Results: Using real-time dynamic observation of target cells, 6 types of intercellular conjunction microtubes were found to function in the transfer of intercellular information between GICs and Mø; GICs and host Mø can fuse into hybrid cells after several rounds of mutual interactions, and then these fusion cells fused with each other; Fusion cells generated offspring cells through symmetrical and asymmetrical division or underwent apoptosis. A "cell in cell" phenomenon was observed in the fusion cells, which was often followed by cell release, namely entosis. Conclusions: Preliminary studies revealed the patterns of cell conjunction via microtubes between GICs and host Mø and the processes of cell fusion, division, and entosis. The results revealed malignant transformation of host Mø, induced by GICs, suggesting complex social relationships among tumor-immune cells in gliomas.
基金资助: Supported by the National Natural Science Foundation of China (Grant No. 81472739) and the Natural Science Foundation of Jiangsu Province (Grant No. BK20151214).
通讯作者:
Jun Dong,E-mail:djdongjun@163.com
E-mail: djdongjun@163.com
引用本文:
Mingxia Zhang, Junjie Chen, Lin Wang, Xiaoyan Ji, Lin Yang, Yujing Sheng, Hairui Liu, Haiyang Wang, Aidong Wang, Xingliang Dai, Xiaonan Li, Qiang Huang, Jun Dong. Preliminary analysis of cellular sociology of co-cultured glioma initiating cells and macrophages in vitro[J]. 临床转化神经科学, 2016, 2(2): 77-86.
Mingxia Zhang, Junjie Chen, Lin Wang, Xiaoyan Ji, Lin Yang, Yujing Sheng, Hairui Liu, Haiyang Wang, Aidong Wang, Xingliang Dai, Xiaonan Li, Qiang Huang, Jun Dong. Preliminary analysis of cellular sociology of co-cultured glioma initiating cells and macrophages in vitro. Translational Neuroscience and Clinics, 2016, 2(2): 77-86.
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