| Original Articles |
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| Generation of genetically modified olfactory ensheathing cells that secrete VEGF:Potential application in cell-based treatment of leukoaraiosis |
| Chuanqiang Qu, Yuanyuan Zhao, Qinjian Sun, Yifeng Du |
| Department of Internal Neurology, Shandong Provincial Hospital, Shandong University, Jinan 250021, China |
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Abstract Objective: We aimed to provide an alternative cell source for cell therapy in leukoaraiosis (LA).
Methods: Olfactory ensheathing cells (OECs) from the olfactory bulb were isolated,cultured,and purified.Next,the lentivirus carrying human VEGF165 gene was constructed and transfected into OECs.
Results: The proliferative capacity of primary OECs was strong.OECs were infected with different multiplicity of infection.The expression level of VEGF was confirmed by real-time PCR with specific primers for GAPDH and VEGF,indicating that the genetically engineered OECs-VEGF produced VEGF with functional activity.
Conclusions: Our data showed that these engineered OECs-VEGF highly express functional VEGF and retain the characteristics of astrocytes and Schwann cells,providing an alternative cell source for cell therapy in LA.
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Received: 13 January 2017
Published: 30 June 2017
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Corresponding Authors:
Chuanqiang Qu,E-mail:quchuanqiang@sina.com
E-mail: quchuanqiang@sina.com
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