Behavioral features of mice fed with a cholesterol-enriched diet:Deficient novelty exploration and unaltered aggressive behavior
Ekaterina Veniaminova1,2, Raymond Cespuglio3,4, Nataliia Markova1,2,5, Niall Mortimer6, Chi Wai Cheung7, Harry W. Steinbusch1, Klaus-Peter Lesch1,6, Tatyana Strekalova1
1 Department of Neuroscience, School for Mental Health and Neuroscience, Maastricht University, Universiteitssingel 40, Maastricht 6200 MD, the Netherlands;
2 Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Baltiyskaya 8, Moscow 125315, Russia;
3 Faculty of Medicine, Neuroscience research Center of Lyon, C. Bernard University,8 Av. Rockefeller, Lyon 69373, France;
4 Department of Anatomy, Guangzhou Medical University, Guangzhou 511436, China;
5 Laboratory of Biomolecular Screening, Institute of Physiologically Active Compounds, Russian Academy of Sciences, Moscow Region, Russia;
6 Division of Molecular Psychiatry, Laboratory of Translational Neuroscience, Department of Psychiatry, Psychosomatics and Psychotherapy, University of Wuerzburg, Fuechsleinstrasse 15, Wuerzburg 97080, Germany;
7 Department of Anaesthesiology, the University of Hong Kong, Queen Mary Hospital, Hong Kong, China
Behavioral features of mice fed with a cholesterol-enriched diet:Deficient novelty exploration and unaltered aggressive behavior
Ekaterina Veniaminova1,2, Raymond Cespuglio3,4, Nataliia Markova1,2,5, Niall Mortimer6, Chi Wai Cheung7, Harry W. Steinbusch1, Klaus-Peter Lesch1,6, Tatyana Strekalova1
1 Department of Neuroscience, School for Mental Health and Neuroscience, Maastricht University, Universiteitssingel 40, Maastricht 6200 MD, the Netherlands;
2 Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Baltiyskaya 8, Moscow 125315, Russia;
3 Faculty of Medicine, Neuroscience research Center of Lyon, C. Bernard University,8 Av. Rockefeller, Lyon 69373, France;
4 Department of Anatomy, Guangzhou Medical University, Guangzhou 511436, China;
5 Laboratory of Biomolecular Screening, Institute of Physiologically Active Compounds, Russian Academy of Sciences, Moscow Region, Russia;
6 Division of Molecular Psychiatry, Laboratory of Translational Neuroscience, Department of Psychiatry, Psychosomatics and Psychotherapy, University of Wuerzburg, Fuechsleinstrasse 15, Wuerzburg 97080, Germany;
7 Department of Anaesthesiology, the University of Hong Kong, Queen Mary Hospital, Hong Kong, China
摘要 Objectives: Previous studies involving mice have demonstrated that a cholesterol-enriched diet evokes liver steatosis, dystrophy, inflammation, and aspects of non-alcoholic fatty liver disease (NAFLD). These changes are accompanied by the activation of pro-inflammatory brain and liver molecular pathways, as well as anxiety and depressive-like behaviors. Given previously reported evidence for the neurobiological relationship between the above-mentioned molecular changes and abnormalities in coping with environmental stimuli, such as interactions with other individuals and new environmental contexts, we hypothesized that novelty exploration and aggressive behavior are affected in a mouse NAFLD model. Methods: To test this hypothesis, young female C57BL/6J mice were fed with a regular chow or a diet containing 0.2% cholesterol for 3 weeks. The mice were then assessed for new object and novel cage exploration, and social interaction in a food competition test. Results: We found reduced object exploration in mice on the cholesterol-enriched diet. This reduction was not related to whether the new object was placed in an anxiogenic or non-anxiogenic environment. These changes were accompanied by diminished exploration of the new environment in a novel cage, and delayed approach to food after a period of food deprivation. Mice on the regular chow or cholesterol-enriched diet showed no differences in aggressive behavior towards a counter-partner in a food competition test. Food intake and body weight did not differ between the groups, thus, excluding their potential as confounders in the measured behaviors. Conclusions: We conclude that a diet enriched with cholesterol reduces novelty exploration irrespective of the anxiogenic level of the environment and does not induce aggressive behavior in female mice.
Abstract: Objectives: Previous studies involving mice have demonstrated that a cholesterol-enriched diet evokes liver steatosis, dystrophy, inflammation, and aspects of non-alcoholic fatty liver disease (NAFLD). These changes are accompanied by the activation of pro-inflammatory brain and liver molecular pathways, as well as anxiety and depressive-like behaviors. Given previously reported evidence for the neurobiological relationship between the above-mentioned molecular changes and abnormalities in coping with environmental stimuli, such as interactions with other individuals and new environmental contexts, we hypothesized that novelty exploration and aggressive behavior are affected in a mouse NAFLD model. Methods: To test this hypothesis, young female C57BL/6J mice were fed with a regular chow or a diet containing 0.2% cholesterol for 3 weeks. The mice were then assessed for new object and novel cage exploration, and social interaction in a food competition test. Results: We found reduced object exploration in mice on the cholesterol-enriched diet. This reduction was not related to whether the new object was placed in an anxiogenic or non-anxiogenic environment. These changes were accompanied by diminished exploration of the new environment in a novel cage, and delayed approach to food after a period of food deprivation. Mice on the regular chow or cholesterol-enriched diet showed no differences in aggressive behavior towards a counter-partner in a food competition test. Food intake and body weight did not differ between the groups, thus, excluding their potential as confounders in the measured behaviors. Conclusions: We conclude that a diet enriched with cholesterol reduces novelty exploration irrespective of the anxiogenic level of the environment and does not induce aggressive behavior in female mice.
Ekaterina Veniaminova, Raymond Cespuglio, Nataliia Markova, Niall Mortimer, Chi Wai Cheung, Harry W. Steinbusch, Klaus-Peter Lesch, Tatyana Strekalova. Behavioral features of mice fed with a cholesterol-enriched diet:Deficient novelty exploration and unaltered aggressive behavior[J]. 临床转化神经科学, 2016, 2(2): 87-95.
Ekaterina Veniaminova, Raymond Cespuglio, Nataliia Markova, Niall Mortimer, Chi Wai Cheung, Harry W. Steinbusch, Klaus-Peter Lesch, Tatyana Strekalova. Behavioral features of mice fed with a cholesterol-enriched diet:Deficient novelty exploration and unaltered aggressive behavior. Translational Neuroscience and Clinics, 2016, 2(2): 87-95.
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